This serological assay may be utilized for functions like characterizing immune responses to viral an an infection by determining the presence of viral antigen-specific antibodies in contaminated and recovered affected particular person sera. At Progressive Evaluation, we provide reliable, fixed merchandise that ship reliable, fixed outcomes.

Molecular Enhancements determine, SARS-CoV-2 IgG Seroconversion ELISA Tools and product amount, SARSCOV2GKT.

This SARS-CoV-2 IgG Seroconversion ELISA Tools from Progressive Evaluation is supposed for the qualitative willpower of SARS-CoV-2 specific antibodies of IgG class in plasma or serum samples. Strip successfully format. Reagents for as a lot as 96 assessments. For evaluation solely. 

This assay has been notably developed using the SARS-CoV-2 receptor binding space (RBD) of the spike protein. This delivers reliable and fixed outcomes with a extreme diploma of reproducibility.Â

This assay could also be carried out in 70 minutes, and has an basic sensitivity of 97-100% and specificity of 100%.

Principle of the Assay:

SARS-CoV-2 specific antibodies will bind to the purified recombinant HEK cell derived receptor-binding space (RBD) of the SARS-CoV-2 spike protein coated on the microtiter plate. After acceptable washing steps, horseradish peroxidase labeled polyclonal anti-human IgG secondary antibody binds to the captured protein. Further secondary antibody is washed away and TMB substrate is used for shade progress at 450 nm. Samples that exceed a determined cut-off price are designated optimistic by this assay.

Further Particulars:

• Detection Aim: SARS-CoV-2 specific antibodies of isotype IgG
• Dilution of Samples: A 1:51 dilution of serum samples is normally beneficial for best outcomes.
• Anticipated Values: Important seroconversion as demonstrated by antibodies specific to every the full-length SARS-CoV-2 spike protein and the RBD space has been detected as early as two days put up symptom onset.
• Sensitivity: 97% for samples obtained larger than 14 days after symptom onset (n=37), 100% for samples obtained larger than 21 days after symptom onset (n=32).
• Specificity: 100% (n=89)
• P.c Settlement: Positive 100%; Damaging 91%
• Storage: Retailer all bundle elements at 4°C upon arrival. Return any unused microplate strips to the plate pouch with desiccant. Reconstituted controls may be saved at -80°C for later use. Retailer all totally different unused bundle elements at 4°C. This bundle should not be used previous the expiration date.

Damaging outcomes do not preclude acute SARS-CoV-2 an an infection. If acute an an infection is suspected, direct testing for SARS-CoV-2 is important. Outcomes from antibody testing should not be used to diagnose or exclude acute SARS-CoV-2 an an infection. Constructive outcomes may be as a consequence of earlier or present an an infection with non-SARS-CoV-2 coronavirus strains, comparable to KHU1, NL63, OC43, or 229E.

bioassay

Nuclear/Cytosol Fractionation Kit - 25 assays
GWB-AXR180	GenWay Biotech	25 assays	Ask for price

Mitochondria/Cytosol Fractionation Kit - 25 assays
GWB-AXR170	GenWay Biotech	25 assays	Ask for price

Nuclear/Cytosol Fractionation Kit
K2111-100	ApexBio	100 assays	EUR 499
Description: Isolates nuclear/cytosol fractions w/o ultracentrifugation

Nuclear/Cytosol Fractionation Kit
K2111-25	ApexBio	25 assays	EUR 301.2

Nuclear/Cytosol Fractionation Kit
KT-388	Kamiya Biomedical Company	100 tests	EUR 564

Nuclear/Cytosol Fractionation Kit
K266-100	Biovision	each	EUR 646.8

Nuclear/Cytosol Fractionation Kit
K266-25	Biovision	each	EUR 314.4

Mitochondria/Cytosol Fractionation Kit
K2103-100	ApexBio	100 assays	EUR 514
Description: Isolates mitochondria/cytosol w/o ultracentrifugation

Mitochondria/Cytosol Fractionation Kit
K2103-25	ApexBio	25 assays	EUR 274
Description: Isolates mitochondria/cytosol w/o ultracentrifugation

Mitochondria/Cytosol Fractionation Kit
KT-148	Kamiya Biomedical Company	100 tests	EUR 544

Mitochondria/Cytosol Fractionation Kit
K256-100	Biovision	each	EUR 614.4

Mitochondria/Cytosol Fractionation Kit
K256-25	Biovision	each	EUR 288

Nuclear/Cytosol Fractionation Kit, Catalog: PI-0107
PI-0107	Akrivis Bio	100 isolations	EUR 500
N/A
Description: To separate nuclear extract from the cytoplasmic fraction in mammalian cells

Nuclear/Cytosolic Fractionation Kit
AKR-171	Cell Biolabs	20 preps	EUR 200

Nuclear/Cytosolic Fractionation Kit
AKR-172	Cell Biolabs	100 preps	EUR 550

Nuclear/Cytosolic Fractionation Kit
MBS168850-20Preps	MyBiosource	20Preps	EUR 340

Nuclear/Cytosolic Fractionation Kit
MBS168850-5x20Preps	MyBiosource	5x20Preps	EUR 1540

Nuclear/Cytosolic Fractionation Kit
MBS168948-100Preps	MyBiosource	100Preps	EUR 740

Nuclear/Cytosolic Fractionation Kit
MBS168948-5x100Preps	MyBiosource	5x100Preps	EUR 3410

Nuclear Fractionation Kit
55R-1307	Fitzgerald	25 assays	EUR 600
Assay Kit containing the folllowing: Cytosol Extraction Buffer A (CEB-A), Cytosol Extraction Buffer B (CEB-B), Nuclear Extraction Buffer (NEB), DTT (1 M), Protease Inhibitor Cocktail (lyophilized)
Description: Nuclear Fractionation Kit for use in the research laboratory

FractionPREP Cell Fractionation Kit
GWB-AXR184	GenWay Biotech	50 assays	Ask for price

FractionPREP? Cell Fractionation Kit
K270-50	Biovision	each	EUR 660

Mitochondria Fractionation Kit
55R-1306	Fitzgerald	25 assays	EUR 567
Assay Kit containing the folllowing: Mitochondria Extraction Buffer, 5X Cytosol Extraction Buffer, DTT (1 M), Protease Inhibitor Cocktail
Description: Mitochondria Fractionation Kit for use in the research laboratory

ReadiPrepâ„¢ Nuclear/Cytoplasmic Fractionation Kit
60000-50Tests	AAT Bioquest	50 Tests	EUR 334
xn
Description: The ReadiPrep™ Nuclear/Cytoplasmic Fractionation Kit offers stepwise isolation of nuclear and cytoplasmic extract from mammalian cells or tissue.

FractionPREP™ Cell Fractionation Kit
K2115-50	ApexBio	50 assays	EUR 398

CytoFrac Cell Fractionation Kit, Catalog: PI-0106
PI-0106	Akrivis Bio	50 isolations	EUR 275
N/A
Description: To provide reproducible separation of four distinct subcellular fractions: Cytoplasmic, Nuclear, Subcellular Membrane/Particulate and Cytoskeletal fractions from a single mammalian sample.

ReadiPrepâ„¢ Mitochondrial/Cytoplasmic Fractionation Kit
60005-50Tests	AAT Bioquest	50 Tests	EUR 334
xn
Description: The ReadiPrepâ„¢ Mitochondrial/Cytoplasmic Fractionation Kit offers a convenient method for isolating mitochondria and cytoplasmic fractions from mammalian cells or tissue.

Minute TM Cytoplasmic and Nuclear Fractionation kit (50 Tests)
SC-003	Inventbiotech	each	EUR 399

TURKEY, GAMMA GLOBULIN, SALT FRACTIONATION, 0.1% AZIDE, 100 MG
7409402-100MG	Lampire Biologicals Laboratories	1MG	EUR 184.22

FractionPREP Cell Fractionation system
55R-1359	Fitzgerald	50 assays	EUR 613
Assay Kit
Description: Cell Fractionation system for use in the research laboratory

MinuteTM Adipose Tissue Fractionation Kit (20 Tests)
AF-023	Inventbiotech	each	EUR 535

CHICKEN ANTI MOUSE IGG, SALT FRACTIONATION, 0.1% AZIDE, 100 MG
7451502-100MG	Lampire Biologicals Laboratories	1MG	EUR 639.56

CHICKEN ANTI SHEEP IGG, SALT FRACTIONATION, 0.1% AZIDE, 100 MG
7451802-100MG	Lampire Biologicals Laboratories	1MG	EUR 639.56

CHICKEN ANTI BOVINE IGG, SALT FRACTIONATION, 0.1% AZIDE, 100 MG
7451102-100MG	Lampire Biologicals Laboratories	1MG	EUR 639.56

Adipose Protein Fractionation Kit (water-soluble/insoluble)
P523-2	101Bio	NULL	Ask for price

Adipose Protein Fractionation Kit (water-soluble/insoluble)
P523-20	101Bio	NULL	Ask for price

Adipose Protein Fractionation Kit (water-soluble/insoluble)
P523-80	101Bio	NULL	Ask for price